Antibodies

Antibodies are proteins that protect you when an unwanted substance enters your body. Produced by your immune system, antibodies bind to these unwanted substances in order to eliminate them from your system. Another word for antibody is immunoglobulin.

CD Markers

CD3

Anti-CD3 antibody

Description

Rabbit monoclonal [SP162] to CD3

Specifications

Concentration –

100 µl at 0.015 mg/ml, 1 ml at 0.015 mg/ml, 500 µl at 0.015 mg/ml

Conjugate –

Unconjugate

Isotype –

IgG

Form –

Liquid.

Storage –

Shipped at 4°C. Store at +4°C. Do Not Freeze.

Anti-CD3 epsilon antibody

Description

Rabbit monoclonal [SP7] to CD3 epsilon

Specifications

Conjugate –

Unconjugated

Isotype –

IgG

Form –

Liquid

Storage –

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Anti-CD3, low endotoxin antibody, Mouse monoclonal

Description

CD3 (cluster of differentiation 3) molecule is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on the surface of all human T lymphocytes.

Specifications

Concentration –

1 mg/mL

Conjugate –

Unconjugated

Isotype –

IgG2a

Form –

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/mg of the protein, as determined by the LAL test.

Storage –

2-8°C

Anti-CD3 Rabbit pAb

Description

Anti-CD3, rabbit polyclonal, recognizes CD3 present in early thymocytes in human and mouse. It is validated for immunohistochemistry in frozen and paraffin sections. Purified rabbit polyclonal antibody. Recognizes CD3. Recognizes CD3 present in early thymocytes.

Specifications

Conjugate –

Unconjugated

Isotype –

IgG

Form –

In proprietary buffer.

Storage –

2-8°C

Anti-CD3 zeta antibody

Description

Rabbit monoclonal [BL-336-1B2] to CD3 zeta

Specifications

Conjugate –

unconjugated

Isotype –

IgG

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Monoclonal Anti-CD3 antibody produced in mouse

Description

Specifications

Concentration –

1 mg/mL

Conjugate –

Unconjugate

Isotype –

IgG1

Form –

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Storage –

2-8°C

Monoclonal Anti-CD3-PE antibody produced in mouse

Description

The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Specifications

Concentration –

0.1 mg/mL

Conjugate –

phycoerythrin (R-PE) conjugate

Isotype –

IgG1

Form –

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Storage –

2-8°C

Monoclonal Anti-CD3−FITC antibody produced in mouse

Description

CD3e molecule, ε (CD3E) is a polypeptide encoded by the CD3E gene in humans. It resides on chromosome 11. The CD3ε cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes and is found in Purkinje cells of the cerebellum as well as in gastric parietal cells of humans and other species. Cytoplasmic cyCD3 expression can only be seen during the early phase of NK-cell differentiation.

Specifications

Conjugate –

FITC conjugate

Isotype –

IgG1

Form –

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage –

2-8°C

CD4

Anti-CD4 antibody

Description

Rabbit monoclonal [EPR6855] to CD4

Specifications

Concentration –

100 µl at 0.143 mg/ml 1 ml at 0.14 mg/ml

Isotype –

IgG

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

Anti-CD4 Antibody (human), APC

Description

The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells.

Specifications

Conjugate –

Allophycocyanin conjugate

Isotype –

IgG2bκ

Form –

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Storage –

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Anti-CD4 (human), PE

Description

The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells.

Specifications

Conjugate –

Anti-CD4 Antibody (human), PE

Description

The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells.

Specifications

Conjugate –

Isotype –

IgG2bκ

Form –

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Storage –

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Anti-CD4 Antibody (mouse)

Description

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells.

Specifications

Concentration –

0.5 mg/mL

Isotype –

IgG2bκ

Form –

Format: Purified Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Storage –

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Anti-CD4 antibody produced in rabbit

Description

Anti-CD4 Antibody detects endogenous levels of total CD4 protein. CD4 (CD4 molecule) is present in the surface of specific leukocytes. CD4 contains four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. It is mapped to human chromosome 12p13.31.

Specifications

Concentration –

~1 mg/mL

Conjugate –

unconjugated

Isotype –

IgG

Form –

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage –

−20°C.

APC Anti-CD4 antibody

Description

APC Mouse monoclonal [MEM-241] to CD4

Specifications

Concentration –

50 tests at 1 mg/m

Conjugate –

APC. Ex: 645nm, Em: 660nm

Isotype –

IgG1

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C.

CD4 Antibody Panel

Description

CD4 Antibody Panel

Specifications

Storage –

Store at -20°C. Please refer to protocols.

FITC Anti-CD4 antibody

Description

FITC Mouse monoclonal [B-A1] to CD4

Specifications

Conjugate –

FITC. Ex: 493nm, Em: 528nm

Isotype –

IgG2a

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C.

Human CD4 ELISA Kit

Description

Human CD4 ELISA Kit

Specifications

Storage –

Store at +4°C. Please refer to protocols.

CD8

Anti-CD8 alpha antibody

Description

Rabbit monoclonal [EPR21769] to CD8 alpha

Specifications

Concentration –

100 µl at 0.491 mg/ml

Conjugate –

Unconjugate

Isotype –

IgG

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Anti-CD8 beta antibody

Description

Rabbit monoclonal [EPR22331-54] to CD8 beta

Specifications

Concentration –

100 µl at 0.63 mg/ml

Isotype –

IgG

Form –

Liquid.

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Anti-CD8 antibody, Rabbit monoclonal

Biotin Anti-IgA antibody

Description

CD8 molecule consists of two chains, termed α and β chain, which are expressed as a disulphide-linked α/β heterdimer or as an α/α homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as α/β heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 α chain (but not β chain).

Specifications

Conjugate –

Unconjugate

Isotype –

IgG

Form –

0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.

Storage –

2-8°C

Biotin Anti-CD8 antibody

Description

Biotin Mouse monoclonal [MEM-31] to CD8

Specifications

Concentration –

100 µg at 1 mg/ml

Conjugate –

Biotin

Isotype –

IgG2a

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Monoclonal Anti-CD8 antibody produced in mouse

Description

The mouse monoclonal antibody LT8 recognizes CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. This antibody blocks Leu2 binding.

Specifications

Concentration –

1 mg/mL

Conjugate –

unconjugated

Form –

Solution in phosphate buffered saline with 15 mM sodium azide.

Storage –

2-8°C

Anti-CD8A antibody produced in mouse

Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

Specifications

Conjugate –

unconjugated

Isotype –

IgG

Form –

Solution in phosphate buffered saline, pH 7.4

Storage –

−20°C

Monoclonal Anti-CD8-FITC antibody produced in mouse

Description

The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).

Specifications

Conjugate –

FITC conjugate

Isotype –

IgG2a

Form –

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Storage –

2-8°C

Monoclonal Anti-CD8-PE antibody produced in mouse

Description

CD8 (cluster of differentiation 8), is a dimeric protein, encoded by the CD8A gene mapped to human chromosome 2p12. Human soluble CD8 (sCD8) is produced by activated CD8+ T lymphocytes.

Specifications

Conjugate –

phycoerythrin (R-PE) conjugate

Isotype –

IgG2a

Form –

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Storage –

2-8°C

Mouse CD8 alpha ELISA Kit

Description

Mouse CD8 alpha ELISA Kit

Specifications

Storage –

Store at +4°C. Please refer to protocols.

PE Anti-CD8 alpha antibody

Description

PE Rat monoclonal [53-6.7] to CD8 alpha

Specifications

Concentration –

100 µg at 0.5 mg/ml

Conjugate –

PE. Ex: 488nm, Em: 575nm

Isotype –

IgG2a

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C. Store In the Dark.

CD25

Anti-CD25 Antibody (human), PE

Description

The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling.

Specifications

Conjugate –

Isotype –

IgG1κ

Form –

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide..

Storage –

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Anti-CD25 antibody, Rabbit monoclonal

Description

IL2RA (interleukin 2 receptor subunit α) gene codes for interleukin-2 receptor α-chain CD25. CD25 is expressed on activated T-cells. The activated T-cells liberate this molecule in the form of a soluble variant (referred to as soluble IL-2 receptor; sIL-2R) from the cell membrane into the circulation. The CD25 gene is located on human chromosome 10p15. The gene spans a length of 48kb with eight exons.

Specifications

Conjugate –

Unconjugated

Isotype –

IgG

Form –

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Storage –

2-8°C

Monoclonal Anti-CD25-Biotin antibody produced in mouse

Description

The antibody MEM-140 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

Specifications

Concentration –

1 mg/mL

Conjugate –

biotin conjugate

Isotype –

IgM

Form –

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Storage –

2-8°C

Monoclonal Anti-CD25-FITC antibody produced in mouse

Description

The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

Specifications

Conjugate –

FITC conjugate

Isotype –

IgG1

Form –

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Storage –

2-8°C

Monoclonal Anti-CD25-PE antibody produced in mouse

Description

Specifications

Conjugate –

phycoerythrin (R-PE) conjugate

Isotype –

IgG1

Form –

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Storage –

2-8°C

CD105

Anti-Endoglin (CD105) Antibody

Description

Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95 kDa subunit. Endoglin is a component of the TGF-β receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-β) family of ligands to signaling receptors.

Specifications

Conjugate –

Unconjugated

Isotype –

IgG1κ

Form –

Format: Purified Protein G Purified Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.e

Storage –

Stable for 1 year at 2-8°C from date of receipt.

Anti-CD105 antibody produced in rabbit

Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium.

Specifications

Concentration –

1.6 mg/mL

Conjugate –

Unconjugate

Form –

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Storage –

−20°C.

FITC Anti-CD105 antibody

Description

FITC Rabbit monoclonal [EPR21846] to CD105

Specifications

Concentration –

100 µl at 0.5 mg/ml 10 µl at 0.5 mg/ml

Isotype –

IgG

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

Human CD105 ELISA Kit

Description

Human CD105 ELISA Kit

Specifications

Storage –

Store at +4°C. Please refer to protocols.

Monoclonal Anti-CD105 antibody produced in mouse

Description

CD105, also known as endoglin (ENG) gene is mapped to human chromosome 9q34.11. The gene codes for a homodimeric transmembrane coreceptor containing disulfide-linked subunits of 95kDa. The encoded protein is mainly expressed on human pre-erythroblasts, macrophages, leukemic cells of the lymphoid and myeloid lineages. It is also present at higher levels on syncytiotrophoblasts of term placenta and vascular endothelial cells.

Specifications

Concentration –

1 mg/mL

Conjugate –

Unconjugate

Isotype –

IgG2a

Form –

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Storage –

2-8°C

Mouse CD105 ELISA Kit

Description

Mouse CD105 ELISA Kit

Specifications

Storage –

Store at +4°C. Please refer to protocols.

Monoclonal Anti-CD105-FITC antibody produced in mouse

Description

The antibody MEM-229 recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Specifications

Conjugate –

FITC conjugate

Isotype –

IgG2a

Form –

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Storage –

2-8°C

Monoclonal Anti-CD105-PE antibody produced in mouse

Description

Specifications

Conjugate –

phycoerythrin (R-PE) conjugate

Isotype –

IgG2a

Form –

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Storage –

2-8°C

Recombinant human CD105 protein

Description

Recombinant human CD105 protein

PE Anti-CD105 antibody

Description

PE Rabbit monoclonal [EPR21846] to CD105

Specifications

Concentration –

100 µl at 0.5 mg/ml 10 µl at 0.5 mg/ml

Conjugate –

PE. Ex: 488nm, Em: 575nm

Isotype –

IgG

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.