Description

DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state

Application

• Enzyme stabilization • Enzymatic reactions • Protein electrophoresis

Description

IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures. IPTG binds to the lac repressor, releasing it from the lac operator, and thereby allowing the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. In addition, IPTG is one of the most efficient molecular inducers for promoter’s transcriptional activity and is used extensively for heterologous protein expression induction in E. coli. Though mainly used in cell culture assays, publications demonstrated the use IPTG in vivo in animal models too.

Applications

• Blue-white screening—induce functional β-galactosidase
• Induce protein expression from inducible T7 RNA polymerase E. coli expression systems egulated by lac operon

Description

X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).

Applications

• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies • Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells • Detection of beta-galactosidase activity in immunological and histochemical procedures

Description

The β-glucuronidase substrate X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronide) is a colorimetric stain for GUS activity, and is used to identify recombinants containing pBACgus vectors, and LIC GUS Control Insert. X-Gluc Solution is provided as a 20 mg/ml stock solution, ready for dilution and use in staining plaques for GUS activity. Each vial contains enough substrate to stain 15 100-mm plates.

Storage

−20°C

Description

Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase.[1] It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

Storage

2-8°C

Description

Superworth Bio is delivering a range of chemicals and reagents for use in the development of diagnostic lateral flow immunoassays.