kits
c dna synthesis kit
Description
The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR.The kit includes Reverse Transcriptase AMV for first strand synthesis, two different primers, our PCR Nucleotide Mix, and Control Neo pa RNA. PCR products that are generated by RT-PCR can be cloned using standard procedures. The amplification of RNA requires the conversion of the RNA substrate into DNA. This is achieved through the use of a reverse transcriptase such as AMV RT (avian myeloblastis virus reverse transcriptase) or M-MuLV RT (moloney murine leukemia virus reverse transcriptase). The resulting cDNA can be used as a template for a standard PCR.
Storage
−20°C
DNA/RNA/Protein Isolation Kits
Description
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast.
Maximum Column Binding Capacity
50 μg for RNA 20 μg for DNA 200 μg for protein
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small RNA (< 200 nt)
Size of DNA Purified
≥ 30 kb
Plasmid Genomics DNA Extraction Kit
Description
This miniprep Plasmid Kit was designed for plasmid DNA purification of cultured bacterial cells. For processing larger volumes.
Binding Capacity
50 µg
Culture Input
1-7 ml
Plasmid Size
1-15 kb
Typical Yield
up to 50 µg
Elution Volume
30-100 µl
PCR Purification/Clean Up Kits
Description
PCR Purification Kit is designed for the rapid purification of PCR Products > 100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. PCR Purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from samples and is compatible with restriction enzyme digestion, ligation into vectors and sequencing.
Binding Capacity
Up to 10 µg
Processing
Manual
Fragment size
100 bp – 10 kb
Elution Volume
>30 µl
One PCR Master Mix
Description
Introducing our cutting-edge One Step RT-PCR Kit, engineered for streamlined RNA analysis in a single reaction. Designed with convenience and accuracy in mind, this kit enables rapid reverse transcription and PCR amplification in a single tube, minimizing handling steps and reducing contamination risks. With optimized enzyme formulations and sensitive detection capabilities, it delivers consistent and reproducible results, making it ideal for a wide range of research and diagnostic applications. Experience seamless RNA analysis with our One Step RT-PCR Kit – revolutionizing your molecular biology workflow.
Biotin Labeling Kits
Description
Biotin Labeling Kit is designed for simple and fast labeling of proteins with biotin. The kit is ideally suited to label antibodies, hormones or other proteins.
Labeling Reagent
750 µg
Dimethylsulfoxide (DMSO)
20 µl
Reaction Buffer (0.1 M sodium phosphate, pH 8.0)
5 ml
Stop Reagent (1 M ethanolamine)
10 µl
Gel Filtration Slurry
5 ml
Agarose Avidin (1:1 slurry)
250 µl
Elisa Kits AND CONSUMABLES
Description
A wide range of ELISA Kits for Human Interleukin and Cytokine , Mouse Rat Porcine , QBlue Assay kits, Bio Assay Kits, Assay Kits, Mitochondria Isolation, Activity, GoldMag Nanoparticle, Total Protein Extraction Kit, Cloning Kits, DNA Extraction Kit Bacteria Identification Kit, QPCR & qRT PCR Products
Size
96
Form
Liquid
Sample Type
Blood
Material
Plastic
miscellaneous
Dntps
Description
The10 mM dNTP Mix is a ready-to-use preparation of ultrapure dATP, dCTP, dGTP, anddTTP (monosodium salts) at a concentration of 10 mM each in sterile deionizedwater at pH 7.0.
Storage
−20°C
Description
The set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. The nucleotide solutions are titrated to pH 7.3-7.5 with NaOH. Since the nucleotides are provided separately, the dNTP Set offers maximum flexibility in preparation of reaction mixes for different applications.
Storage
−20°C
Taq DNA Polymerase
Description
Taq DNA polymerase is a thermostable enzyme isolated from E. coli which encodes Taq DNA polymerase gene. This enzyme contains 5’-3’ poly merase and 5’-3’ exonuclease activity .
Storage Buffer
50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF , 50% glycerol.
Application
• Amplification of DNA fragments up to 5 kb
• Labeling of PCR products with modified nucleotides (biotin-dUTP, fluorescein-dUTP)
• Cycle sequencing
• Generation of PCR product for TA cloning
sybr green kit
Description
The SYBR® Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), JumpStart™ Taq DNA polymerase, and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
Storage
−20°C