Antibiotics
An antibiotic is a type of antimicrobial substance active against bacteria. It is the most important type of antibacterial agent for fighting bacterial infections, and antibiotic medications are widely used in the treatment and prevention of such infections. They may either kill or inhibit the growth of bacteria.
Blasticidin S HCl (10 mg/mL)
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian cells and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.
Specifications
Cell Type –
Eukaryotic Cells, Prokaryotic Cells
Concentration –
10 mg/mL
Culture Type –
Mammalian Cell Culture, Insect Cell Culture
Form –
Liquid
Product Type –
Antibiotic
Shelf Life –
9 Months
Sterility –
Sterile-filtered
With Additives
–
HEPES
Contents & Storage
Storage conditions:
-5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture
Blasticidin S HCl, powder
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.
Specifications
Color –
White, off-white
Cell Type –
Eukaryotic Cells, Prokaryotic Cells
Concentration –
50 to 100 μg/mL
Culture Type –
Mammalian Cell Culture, Insect Cell Culture
Form –
Powder
Product Type –
Antibiotic
Shelf Life –
48 Months
Sterility –
Sterile
With Additives–
HEPES
For Use With (Application) –
Bacterial Selection
Contents & Storage
Storage conditions:
-5°C to -30°C
Shipping conditions:
room temperature
Shelf life: 48 months from date of manufacture
Geneticin Selective Antibiotic (G418 Sulfate) (50 mg/mL)
Geneticin Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 – 200 µg/ml for bacteria, or 200 – 500 µg/ml for most mammalian cells. This product is supplied as a 50 mg/ml solution in water. We offer a variety of cell culture antibiotics for your convenience.
Specifications
Cell Type –
Eukaryotic Cells, Prokaryotic Cells
Concentration –
50 mg/mL
Culture Type –
Mammalian Cell Culture, Insect Cell Culture
Form –
Liquid
Product Type –
Antibiotic
Shelf Life –
24 Months
Sterility –
Sterile-filtered
For Use With (Application)
– Eukaryotic Selection⁄Stable Cell Line Generation
Product Line –
Geneticin
Quantity –
20 mL
Shipping Condition –
Room Temperature
Contents & Storage
Storage conditions:
2 to 8°C
Shipping conditions:
Ambient
Shelf life: 24 months from date of manufacture
Geneticin Selective Antibiotic (G418 Sulfate), Powder
Geneticin Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin™ Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 – 200 µg/ml for bacteria, or 200 – 500 µg/ml for most mammalian cells. This product is supplied as a powder and should be made into a stock solution of 10 – 50 mg/ml in water. We offer a variety of cell culture antibiotics for your convenience
Specifications
Cell Type –
Eukaryotic Cells, Prokaryotic Cells
Concentration –
10 to 50 μg/mL
Form –
Powder
Product Type –
Antibiotic
Shelf Life –
24 Months
Sterility –
Sterile
For Use With (Application) –
Eukaryotic Selection⁄Stable Cell Line Generation
Product Line –
Geneticin
Quantity –
25 g
Shipping Condition –
Room Temperature
Contents & Storage
Storage conditions: 15 to 30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
Geneticin Selective Antibiotic (G418 Sulfate), Powder
Geneticin Selection Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin Selection Antibiotic (G418 Sulfate) is conferred by the E. coli APH(3’)–I and APH(3’)–II resistance genes. Geneticin Selection Antibiotic (G418 Sulfate) is used as a selection antibiotic in the concentration range of 100–200 µg/mL for bacteria, or 200–500 µg/mL for most mammalian cells. This product is supplied as a powder and should be made into a stock solution of 10–50 mg/mL in water.
Specifications
Cell Type –
Eukaryotic Cells, Prokaryotic Cells
Form –
Powder
Product Type –
Antibiotic
Shelf Life –
24 Months
For Use With (Application) –
Eukaryotic Selection⁄Stable Cell Line Generation
Product Line –
Geneticin
Shipping Condition –
Room Temperature
Contents & Storage
Storage conditions: 15 to 30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
Kanamycin Sulfate
Kanamycin Sulfate is a water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus. Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco™ Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria, and is used for the prevention of bacterial contamination of cell cultures. This product is provided as a 10 mg/ml stock solution. The recommended working concentration for cell culture is 100 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.
Specifications
Type – Kanamycin
Physical Form – Liquid
Sterile Filtered – Yes
Concentration – 100X
Quantity – 100 mL
Validated Application – Bacterial Selection
Shipping Condition –
Dry Ice
Green Features – Sustainable packaging
Contents & Storage
Storage conditions: -5 to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture
Kanamycin Sulphate (100X)
Kanamycin Sulfate is a water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus. Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco™ Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria, and is used for the prevention of bacterial contamination of cell cultures. This product is provided as a 10 mg/ml stock solution. The recommended working concentration for cell culture is 100 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.
Specifications
Type – Kanamycin
Physical Form – Liquid
Sterile Filtered – Yes
Concentration – 100X
Quantity – 20×100 mL
Validated Application – Bacterial Selection
Green Features – Sustainable packaging
Contents & Storage
Storage conditions: -5 to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture
Puromycin Dihydrochloride
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene. Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8) in 10 vials each containing 1 mL.
Specifications
Concentration – 10 mg/mL
Form – Liquid
Product Type – Antibiotic
Shelf Life – 12 Months
Sterility – Sterile-filtered
With Additives – HEPES
For Use With (Application) – Eukaryotic Selection⁄Stable Cell Line Generation
Quantity – 10 vials
Shipping Condition – Dry Ice
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture
Zeocin Selection Reagent
Zeocin Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. Zeocin Selection Antibiotic acts by binding to and cleaving DNA, leading to cell death. Zeocin Selection Antibiotic is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures. It is used at a working concentration ranging between 50–400 µg/mL depending on the cell type. This product is supplied at 100 mg/mL in sterile water in one 50 mL bottle.
Specifications
Concentration –
100 mg/mL
Culture Type –
Mammalian Cell Culture, Insect Cell Culture
Form –
Liquid
Product Type –
Antibiotic
Shelf Life –
12 Months
Sterility –
Sterile-filtered
For Use With (Application) –
Eukaryotic Selection/Stable Cell Line Generation, Bacterial Selection
Product Line –
Zeocin
Quantity –
50mL
Contents & Storage
Storage conditions: -5 to -20°C
Shipping conditions: Ice